7PU4

Crystal structure of the dimer RBP-N and RBP-Trunc from Thermotoga maritima Ribose Binding Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-26
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	65.210, 84.200, 103.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.010 - 1.690
R-factor	0.2073
Rwork	0.206
R-free	0.23990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fn9
RMSD bond length	0.008
RMSD bond angle	0.892
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.010	1.750
High resolution limit [Å]	1.690	1.690
Rmerge	0.122	1.800
Rmeas	0.127	1.196
Rpim	0.035	1.104
Number of reflections	64629	6298
<I/σ(I)>	14.25	0.96
Completeness [%]	99.8	98.39
Redundancy	13.3	12.8
CC(1/2)	0.999	0.318

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	0.1 M HEPES pH: 7.5, PEG 20000 15% w/v, RBP-N and RBP-Trunc equimolarly mixed at 0.5 mM