7PU0
Crystal Structure of Two-Domain Laccase mutant H165A/M199G from Streptomyces griseoflavus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | RIGAKU MICROMAX-003 |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2020-12-18 |
Detector | RIGAKU HyPix-6000HE |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.235, 94.591, 120.867 |
Unit cell angles | 90.00, 91.39, 90.00 |
Refinement procedure
Resolution | 24.740 - 2.200 |
R-factor | 0.1585 |
Rwork | 0.157 |
R-free | 0.18850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lhl |
RMSD bond length | 0.007 |
RMSD bond angle | 0.890 |
Data reduction software | CrysalisPro |
Data scaling software | CrysalisPro |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.190 | 1.070 |
Number of reflections | 85543 | 4591 |
<I/σ(I)> | 6.1 | 1.5 |
Completeness [%] | 98.1 | 98.7 |
Redundancy | 3.7 | |
CC(1/2) | 0.980 | 0.520 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 296 | 15 % v/v PEG Smear High, 0.15 M Ammonium acetate, 0.1 M Sodium citrate |