7PSO

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with Avadomide (CC-122)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	56.562, 60.162, 88.406
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.240 - 1.520
R-factor	0.1861
Rwork	0.184
R-free	0.21690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v2y
RMSD bond length	0.012
RMSD bond angle	1.862
Data reduction software	XDS
Data scaling software	XDS
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.240	1.610
High resolution limit [Å]	1.520	1.520
Rmerge	0.066	1.337
Rmeas	0.069	1.392
Number of reflections	47297	7506
<I/σ(I)>	18.96
Completeness [%]	99.9	99.4
Redundancy	12	12
CC(1/2)	0.999	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.5 M (NH4)H2PO4