7PSH
Crystal structure of beta-glucuronidase from Acidobacterium capsulatum at 1.24 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-15 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.911880 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 82.865, 44.149, 136.277 |
Unit cell angles | 90.00, 97.58, 90.00 |
Refinement procedure
Resolution | 74.690 - 1.240 |
Rwork | 0.169 |
R-free | 0.18780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5g0m |
RMSD bond length | 0.018 |
RMSD bond angle | 1.959 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.690 | 1.260 |
High resolution limit [Å] | 1.240 | 1.260 |
Number of reflections | 134306 | 6247 |
<I/σ(I)> | 11.6 | 2.2 |
Completeness [%] | 97.2 | |
Redundancy | 6.1 | |
CC(1/2) | 0.998 | 0.794 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.5 M AmSO4 1 M LiSO4 0.1 M Trisodium Citrate |