7PRX
wildtype ligand binding domain of the glucocorticoid receptor complexed with velsecorat and a PGC1a coactivator fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.972422 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.342, 73.433, 43.933 |
| Unit cell angles | 90.00, 105.86, 90.00 |
Refinement procedure
| Resolution | 42.260 - 2.200 |
| R-factor | 0.2286 |
| Rwork | 0.226 |
| R-free | 0.28080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m2z |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.430 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.124 | 0.694 |
| Rpim | 0.081 | 0.466 |
| Number of reflections | 13112 | 1111 |
| <I/σ(I)> | 6.6 | 1.7 |
| Completeness [%] | 99.2 | 99 |
| Redundancy | 3.3 | 3.1 |
| CC(1/2) | 0.995 | 0.709 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 18 % PEG8000, 2 % 2-Propanol, 0.1 M Sodium acetate, 0.1 M HEPES pH 7.5 |
