7PRN

E1M, D97A, E99A MUTANT OF RH. BLASTICA PORIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-11
Detector	MARRESEARCH
Spacegroup name	H 3
Unit cell lengths	104.400, 104.400, 124.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.900 - 2.250
R-factor	0.215 *
Rwork	0.215
R-free	0.22200
Structure solution method	DIFFERENCE FOURIER
Starting model (for MR)	1prn
RMSD bond length	0.020
RMSD bond angle	0.038
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.950	2.370
High resolution limit [Å]	2.250	2.250
Rmerge	0.044	0.132
Number of reflections	23933	3524 *
<I/σ(I)>	30.2	11.4
Completeness [%]	99.8	100 *
Redundancy	3.5	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2 *	20 *	Kreusch, A., (1994) J.Mol.Biol., 243, 891. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop		300 (mM)
4	1	drop	n-octyltetraoxyethylene	0.6 (%(w/v))
5	1	drop		3 (mM)
6	1	drop	PEG600	10-18 (%)
7	1	reservoir	PEG600	30-38 (%(w/v))