7PQS
SRPK1 in complex with MSC2711186
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-26 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 65 |
| Unit cell lengths | 75.132, 75.132, 309.709 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.860 - 2.200 |
| R-factor | 0.2008 |
| Rwork | 0.199 |
| R-free | 0.23560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mxx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.620 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.810 | 49.810 | 2.270 |
| High resolution limit [Å] | 2.200 | 9.070 | 2.200 |
| Rmerge | 0.173 | 0.065 | 1.676 |
| Rmeas | 0.177 | 0.066 | 1.723 |
| Rpim | 0.039 | 0.014 | 0.399 |
| Total number of observations | 1026667 | 15985 | 80156 |
| Number of reflections | 50020 | 743 | 4334 |
| <I/σ(I)> | 12.5 | 38.1 | 2 |
| Completeness [%] | 100.0 | 99.5 | 100 |
| Redundancy | 20.5 | 21.5 | 18.5 |
| CC(1/2) | 0.998 | 0.999 | 0.723 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 278 | 15% PEG Smear Broad, 0.1M cacodylate, 5% tacsimate, 10% ethylene glycol |
