7PQA
Crystal Structure of the Ring Nuclease 0811 mutant-S12G/K169G from Sulfolobus islandicus (Sis0811)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9779 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.930, 104.970, 78.306 |
| Unit cell angles | 90.00, 102.89, 90.00 |
Refinement procedure
| Resolution | 43.932 - 2.040 |
| Rwork | 0.194 |
| R-free | 0.23790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7pq2 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.860 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.380 | 2.070 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Number of reflections | 38798 | 1925 |
| <I/σ(I)> | 18.5 | |
| Completeness [%] | 97.6 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.990 | 0.839 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350, 0.1M Bis-Tris propane pH 7.5, 0.2M KSCN |
