7PQ6
Crystal Structure of the Ring Nuclease 0811 mutant-S12A from Sulfolobus islandicus (Sis0811)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 77.054, 103.735, 77.678 |
Unit cell angles | 90.00, 102.99, 90.00 |
Refinement procedure
Resolution | 60.820 - 2.670 |
Rwork | 0.220 |
R-free | 0.27950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7pq2 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.602 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.820 | 2.714 |
High resolution limit [Å] | 2.670 | 2.670 |
Number of reflections | 16673 | 3165 |
<I/σ(I)> | 6.7 | |
Completeness [%] | 98.0 | |
Redundancy | 6.3 | |
CC(1/2) | 0.945 | 0.494 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350, 0.1M Bis-Tris propane pH 7.5, 0.2M KSCN |