7PPZ
Crystal structure of the Burkholderia Lethal Factor 1 (BLF1) C94S inactive mutant in complex with human eIF4A - Crystal form A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.980, 50.360, 95.740 |
| Unit cell angles | 90.00, 111.92, 90.00 |
Refinement procedure
| Resolution | 63.070 - 2.520 |
| R-factor | 0.2053 |
| Rwork | 0.202 |
| R-free | 0.27610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3TUA and 2ZU6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.675 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 63.070 | 63.070 | 2.590 |
| High resolution limit [Å] | 2.520 | 11.270 | 2.520 |
| Rmerge | 0.163 | 0.036 | 0.725 |
| Rmeas | 0.190 | 0.042 | 0.852 |
| Rpim | 0.096 | 0.022 | 0.441 |
| Total number of observations | 76647 | 803 | 5285 |
| Number of reflections | 20378 | 244 | 1457 |
| <I/σ(I)> | 8.1 | 30.2 | 1.7 |
| Completeness [%] | 99.2 | 94.9 | 98.7 |
| Redundancy | 3.8 | 3.3 | 3.6 |
| CC(1/2) | 0.985 | 0.998 | 0.703 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | 0.1 M MES, 50 mM magnesium chloride, 10 % (w/v) 2-propanol, 5 % (w/v) PEG 4000 |
