7PPR
The structure of UDP-glucose pyrophosphorylase from Aspergillus fumigatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9281 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 134.160, 152.610, 160.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.000 - 2.570 |
| R-factor | 0.2295 |
| Rwork | 0.227 |
| R-free | 0.27740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i5k |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.641 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.010 | 2.650 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Number of reflections | 52428 | 4513 |
| <I/σ(I)> | 15.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.990 | 0.514 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.8 M lithium sulphate monohydrate, and 0.1 M sodium acetate trihydrate, pH 4.6 |
