7PPA
High resolution structure of bone morphogenetic protein receptor type II (BMPRII) extracellular domain in complex with BMP10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-13 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.91589 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.718, 47.228, 250.085 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 125.040 - 1.480 |
Rwork | 0.165 |
R-free | 0.20660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6sf3 2hlq |
RMSD bond length | 0.015 |
RMSD bond angle | 1.871 |
Data reduction software | DIALS (1.14.5) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 125.040 | 1.510 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmerge | 0.111 | 1.850 |
Rmeas | 0.115 | 1.933 |
Rpim | 0.032 | 0.554 |
Number of reflections | 89787 | 4320 |
<I/σ(I)> | 11.2 | 1.4 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 12.7 | 11.7 |
CC(1/2) | 0.999 | 0.590 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 294.15 | 14% PEG 3350 0.19 M Ammonium citrate dibasic 0.02 M Sodium citrate tribasic dihydrate |