7PP2
Complex of rice blast (Magnaporthe oryzae) effector protein AVR-Pii with the host target Exo70F2 from Rice (Oryza sativa)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-05 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.316, 76.621, 67.873 |
| Unit cell angles | 90.00, 107.76, 90.00 |
Refinement procedure
| Resolution | 66.560 - 2.690 |
| R-factor | 0.253 |
| Rwork | 0.252 |
| R-free | 0.27920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rl5 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.187 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.810 | 2.820 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmeas | 0.156 | |
| Number of reflections | 19153 | 2534 |
| <I/σ(I)> | 8.4 | 1.3 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 6.7 | 6.5 |
| CC(1/2) | 0.997 | 0.541 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.3 M Ammonium iodide; 30% v/v PEG3350 |
