7POW
Crystal structure of phosphatidyl serine synthase (PSS) in transition state.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9796 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.350, 61.220, 79.310 |
| Unit cell angles | 90.00, 118.72, 90.00 |
Refinement procedure
| Resolution | 38.040 - 2.510 |
| R-factor | 0.2185 |
| Rwork | 0.215 |
| R-free | 0.27570 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.040 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.150 | 0.290 |
| Rmeas | 0.160 | 0.310 |
| Rpim | 0.050 | 0.120 |
| Number of reflections | 30244 | 3027 |
| <I/σ(I)> | 9.2 | 4.2 |
| Completeness [%] | 98.4 | 88.9 |
| Redundancy | 5.4 | 3.44 |
| CC(1/2) | 0.987 | 0.966 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 300 | Qiagens MBclass Suit and MBclass Suit II Molecular Dimension MemGold |
