7POH

Crystal structure of ZAD-domain of Serendipity-d protein from D.melanogaster

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 43 21 2
Unit cell lengths	99.161, 99.161, 117.667
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.120 - 2.850
R-factor	0.2187
Rwork	0.218
R-free	0.24040
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	2.542
Data reduction software	HKL-2000
Data scaling software	Aimless (0.7.7)
Phasing software	SHELX
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	99.160	99.160	3.000
High resolution limit [Å]	2.850	9.010	2.850
Rmerge	0.089	0.046	0.853
Rmeas	0.100	0.053	0.961
Rpim	0.045	0.025	0.431
Total number of observations	57927
Number of reflections	12833	444	1874
<I/σ(I)>	9
Completeness [%]	91.3	86.9	92.9
Redundancy	4.5	4.2	4.7
CC(1/2)	0.994	0.990	0.649

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	288	0.1M HEPES pH 7.5, 1.3M Lithium sulfate 1.3M, 0.1M Sodium chloride