7PO9
Crystal structure of ZAD-domain of M1BP protein from D.melanogaster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.853, 48.827, 141.586 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.790 - 1.900 |
| R-factor | 0.2434 |
| Rwork | 0.241 |
| R-free | 0.28630 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.095 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 141.590 | 141.590 | 1.730 |
| High resolution limit [Å] | 1.700 | 9.000 | 1.700 |
| Rmerge | 0.059 | 0.032 | 2.275 |
| Rmeas | 0.061 | 0.034 | 2.470 |
| Rpim | 0.018 | 0.011 | 0.929 |
| Total number of observations | 250275 | 1957 | 6248 |
| Number of reflections | 21730 | 204 | 919 |
| <I/σ(I)> | 13.6 | 114.6 | 0.2 |
| Completeness [%] | 97.9 | 99.4 | 80.6 |
| Redundancy | 11.5 | 9.6 | 6.8 |
| CC(1/2) | 0.999 | 0.999 | 0.677 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.2M Calcium Acetate, 0.1MES pH 6.0, 20% PEG8000 |
