7PO7

Phosphoglycolate phosphatase from Mus musculus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.972422
Spacegroup name	P 1
Unit cell lengths	84.262, 91.197, 101.317
Unit cell angles	103.46, 89.98, 104.63

Refinement procedure

Resolution	19.970 - 2.310
R-factor	0.2085
Rwork	0.208
R-free	0.22960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bkm
RMSD bond length	0.003
RMSD bond angle	0.789
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.170	2.540
High resolution limit [Å]	2.310	2.310
Rmerge	0.094	1.078
Rpim	0.062	0.684
Number of reflections	88859	4443
<I/σ(I)>	7.43	1.23
Completeness [%]	71.3	14.39
Redundancy	3.1	3.4
CC(1/2)	0.993	0.281

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M Tris-HCl 0.2 M (NH4)2SO4 12% (w/v) PEG 8000