7PNC

Dark state structure of Sensory Rhodopsin II solved by serial millisecond crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	298
Detector technology	PIXEL
Collection date	2019-05-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000342
Spacegroup name	C 2 2 21
Unit cell lengths	89.750, 131.700, 51.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.440 - 2.200
R-factor	0.1841
Rwork	0.183
R-free	0.20090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h68
RMSD bond length	0.004
RMSD bond angle	0.802
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.440	2.140
High resolution limit [Å]	2.100	2.110
Number of reflections	18087	1722
<I/σ(I)>	5.56
Completeness [%]	100.0
Redundancy	242
CC(1/2)	0.991	0.310

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		295	CaCl 150mM, Glycine 100mM, 38%(v/v) PEG 400, pH 7.5