7PLQ

Crystal structure of the PARP domain of wheat SRO1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-31
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	78.513, 34.373, 132.581
Unit cell angles	90.00, 101.22, 90.00

Refinement procedure

Resolution	43.350 - 2.130
R-factor	0.2167
Rwork	0.214
R-free	0.26560
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.350	2.260
High resolution limit [Å]	2.130	2.130
Rmeas	0.019	0.166
Number of reflections	37083	5558
<I/σ(I)>	8.69	1.1
Completeness [%]	97.3	89.7
Redundancy	6.97	6.28
CC(1/2)	0.996	0.416

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.8	293	0.1 MES, 0.2 M ammonium sulfate, 17% (w/v) PEG 3350