7PL7

Crystal structure of yeast Otu2 OTU domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-16
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.979
Spacegroup name	I 21 21 21
Unit cell lengths	44.669, 60.055, 148.349
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.772 - 2.600
R-factor	0.2271
Rwork	0.225
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bou
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.772	42.772	2.750
High resolution limit [Å]	2.590	7.660	2.590
Rmerge	0.132	0.043	1.002
Rmeas	0.142	0.047	1.080
Total number of observations	84923
Number of reflections	11976	469	1925
<I/σ(I)>	13.95	39.32	2.35
Completeness [%]	99.8	99.4	98.5
Redundancy	7.091	6.746	7.197
CC(1/2)	0.998	0.999	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.2 M KSCN 0.1 M BisTris propane pH 6.5 20% PEG 3350 20% glycerol