7PL2

Crystal structure of choline-binding module of LytB from Streptococcus pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979312
Spacegroup name	P 6
Unit cell lengths	202.100, 202.100, 26.468
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.760 - 2.980
R-factor	0.2215
Rwork	0.219
R-free	0.27250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cnl
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.540	3.160
High resolution limit [Å]	2.980	2.980
Rmerge	0.151	0.621
Rpim	0.117	0.467
Number of reflections	12892	2036
<I/σ(I)>	4.2	1.5
Completeness [%]	97.1
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	Ammonium acetate Bis-Tris propane