7PKJ
Streptococcus pyogenes apo GapN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.918401 |
| Spacegroup name | P 1 |
| Unit cell lengths | 97.749, 99.893, 106.182 |
| Unit cell angles | 77.67, 75.19, 67.12 |
Refinement procedure
| Resolution | 20.030 - 1.989 |
| R-factor | 0.1942 |
| Rwork | 0.192 |
| R-free | 0.23190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1euh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.148 | 2.170 |
| High resolution limit [Å] | 1.989 | 1.989 |
| Rmerge | 0.304 | 1.002 |
| Rmeas | 0.373 | 1.201 |
| Rpim | 0.213 | 0.655 |
| Number of reflections | 153369 | 7668 |
| <I/σ(I)> | 3.195 | |
| Completeness [%] | 87.5 | 70.72 |
| Redundancy | 2.91 | 3.23 |
| CC(1/2) | 0.847 | 0.396 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.15 | 0.2 M Lithium sulfate 0.1 M Bis-Tris pH 5.5 25 % PEG 3350 |
