7PJK
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with a benzotriazole analog of thalidomide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-09 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.779, 59.435, 88.189 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.440 - 1.990 |
| R-factor | 0.2107 |
| Rwork | 0.208 |
| R-free | 0.25440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v2y |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.305 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.440 | 2.110 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.095 | 1.810 |
| Number of reflections | 21052 | 3305 |
| <I/σ(I)> | 14.9 | 1.25 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 12.8 | 13 |
| CC(1/2) | 0.999 | 0.583 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.4 M (NH4)2HPO4 |
