7PJK

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with a benzotriazole analog of thalidomide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-09
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	56.779, 59.435, 88.189
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.440 - 1.990
R-factor	0.2107
Rwork	0.208
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v2y
RMSD bond length	0.003
RMSD bond angle	1.305
Data reduction software	XDS
Data scaling software	XDS
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.440	2.110
High resolution limit [Å]	1.990	1.990
Rmerge	0.095	1.810
Number of reflections	21052	3305
<I/σ(I)>	14.9	1.25
Completeness [%]	99.7	98.3
Redundancy	12.8	13
CC(1/2)	0.999	0.583

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.4 M (NH4)2HPO4