7PIZ
The structure of phosphoglucomutase from Candida albicans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-17 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.950, 86.841, 109.920 |
| Unit cell angles | 90.00, 92.80, 90.00 |
Refinement procedure
| Resolution | 25.710 - 2.150 |
| R-factor | 0.1986 |
| Rwork | 0.196 |
| R-free | 0.25890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | A homology model based on the orthologue protein from human (PDB 5EPC)) |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.710 | 2.200 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 65230 | 4312 |
| <I/σ(I)> | 4.5 | |
| Completeness [%] | 95.5 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.979 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 200 mM lithium sulphate monohydrate, 100 mM Tris-HCl pH 8.5, 30% w/v polyethylene glycol 4,000 |
