7PI7
PfCyRPA bound to monoclonal antibody Cy.002 Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-04 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 205.050, 80.690, 117.360 |
| Unit cell angles | 90.00, 108.73, 90.00 |
Refinement procedure
| Resolution | 37.920 - 2.720 |
| R-factor | 0.274 |
| Rwork | 0.273 |
| R-free | 0.30050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tih |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4 (20-APR-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.550 | 2.810 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Number of reflections | 169805 | 15709 |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 99.6 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.999 | 0.864 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M lithium sulfate, 30 % polyvinylpyrrolidone K15, 0.1 M HEPES pH7 |
