7PHV
PfCyRPA bound to monoclonal antibody Cy.007 Fab fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.097, 87.000, 342.774 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.240 - 3.091 |
R-factor | 0.2892 |
Rwork | 0.287 |
R-free | 0.33210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5tih |
RMSD bond length | 0.008 |
RMSD bond angle | 0.950 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4 (20-OCT-2021)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.970 | 3.220 |
High resolution limit [Å] | 3.090 | 3.090 |
Number of reflections | 564609 | 62331 |
<I/σ(I)> | 5.1 | |
Completeness [%] | 99.9 | |
Redundancy | 13.5 | |
CC(1/2) | 0.969 | 0.733 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M zinc acetate, 10 % PEG 3000, 0.1 M sodium acetate pH 4.5 |