7PGY

Structure of light-adapted AsLOV2 wild type

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	35.610, 56.010, 66.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.845 - 1.090
Rwork	0.123
R-free	0.16120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v0u
RMSD bond length	0.012
RMSD bond angle	1.821
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.845	1.160
High resolution limit [Å]	1.090	1.090
Number of reflections	55866	8789
<I/σ(I)>	12
Completeness [%]	99.2	98
Redundancy	7	6.2
CC(1/2)	0.999	0.450

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.8	298	0.1 M sodium acetate pH 4.6-5.0, 6-8% (w/v) PEG 4000, 30% (v/v) glycerol