7PGL

HHIP-N, the N-terminal domain of the Hedgehog-Interacting Protein (HHIP), apo-form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96862
Spacegroup name	P 43 21 2
Unit cell lengths	59.050, 59.050, 98.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.670 - 2.630
R-factor	0.2452
Rwork	0.244
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HHIP-N:SOS
RMSD bond length	0.002
RMSD bond angle	0.538
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2140: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.670	2.700
High resolution limit [Å]	2.630	2.630
Rmerge	0.058
Rpim	0.024
Number of reflections	5640	420
<I/σ(I)>	22.3	1.4
Completeness [%]	100.0	100
Redundancy	12.3	1.4
CC(1/2)	1.000	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M HEPES pH 7.0, 0.2 M ammonium chloride, 0.02 M hexamine cobalt (III) chloride and 20% w/v PEG 6000