7PGF
Calcium-selective Sp1 channel pore domain only
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-01 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 133.682, 133.682, 130.744 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.930 - 3.500 |
| R-factor | 0.2658 |
| Rwork | 0.265 |
| R-free | 0.28970 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.449 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.622 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.156 | 1.000 |
| Number of reflections | 9000 | 879 |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 97.2 | |
| Redundancy | 11.5 | |
| CC(1/2) | 0.995 | 0.126 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | CaVSp1p purified in DM was concentrated to 13 mg mL-1 and reconstituted in bicelles prior crystallization to a final bicelle concentration of 8%. Native crystals grew in 25% PEG4000, 200 mM MgCl2, 100 mM MES, pH 6.5 |
