7PG7

10-decarboxylase TamK from Streptomyces tsukubaensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.87313
Spacegroup name	C 1 2 1
Unit cell lengths	122.835, 39.692, 97.977
Unit cell angles	90.00, 114.08, 90.00

Refinement procedure

Resolution	28.040 - 1.510
R-factor	0.2003
Rwork	0.199
R-free	0.22110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tw2
RMSD bond length	0.007
RMSD bond angle	0.991
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.100	1.540
High resolution limit [Å]	1.510	1.510
Rmerge	0.041	0.693
Number of reflections	65700	2707
<I/σ(I)>	15.4
Completeness [%]	96.6
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293.15	0.04M potassium phosphate monobasic, 16% PEG 8000, 20% glycerol