7PF7

Apo structure of SynFtn variant D65A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	F 4 3 2
Unit cell lengths	177.140, 177.140, 177.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.639 - 1.700
R-factor	0.1829
Rwork	0.182
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 5OUW
RMSD bond length	0.005
RMSD bond angle	0.712
Data reduction software	DIALS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	53.330	53.330	1.730
High resolution limit [Å]	1.690	7.560	1.690
Rmerge	0.070	0.022	4.264
Rmeas	0.071	0.023	4.292
Rpim	0.008	0.003	0.491
Total number of observations	2119682	24247	146252
Number of reflections	27095	388	1939
<I/σ(I)>	42.4	167.6	1.5
Completeness [%]	100.0	98.7	100
Redundancy	78.2	62.5	75.4
CC(1/2)	1.000	1.000	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	289	2M NaCl