7PED

DEPTOR DEP domain tandem (DEPt)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	50.862, 99.014, 68.245
Unit cell angles	90.00, 109.68, 90.00

Refinement procedure

Resolution	53.903 - 1.930
R-factor	0.2113
Rwork	0.211
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f7z
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.010	1.980
High resolution limit [Å]	1.930	1.930
Rmerge	0.033	1.132
Number of reflections	46594	2829
<I/σ(I)>	14.2
Completeness [%]	97.4
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	PEG3350,natrium hydrochlorid