7PCK
CRYSTAL STRUCTURE OF WILD TYPE HUMAN PROCATHEPSIN K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.700, 84.400, 155.600 |
| Unit cell angles | 90.00, 90.30, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.200 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cjl |
| RMSD bond length | 0.041 |
| RMSD bond angle | 2.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.120 | 0.260 |
| Total number of observations | 121990 * | |
| Number of reflections | 21801 | |
| <I/σ(I)> | 10 | 3 |
| Completeness [%] | 87.2 | 68 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 100 MM TRIS-HCL, 5% 2-METHYL-2,4-PENTANEDIOL, 12 MM AMMONIUM SULPHATE AND 9% PEG, pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10.7 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 3 | 1 | reservoir | MPD | 5 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 12 (mM) | |
| 5 | 1 | reservoir | PEG3350 | 9 (%) |
