7PCA

Functional and structural characterization of redox sensitive superfolder green fluorescent protein and variants

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	36.870, 47.370, 117.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.850 - 1.050
R-factor	0.15
Rwork	0.149
R-free	0.16250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qyo
RMSD bond length	0.011
RMSD bond angle	1.216
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.850	1.088
High resolution limit [Å]	1.050	1.050
Rmerge	0.044
Rpim	0.023
Number of reflections	89058	4957
<I/σ(I)>	16.24
Completeness [%]	91.7	51.7
Redundancy	4.4	2.1
CC(1/2)	0.999	0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	295	46% ETOH, 0.25% v/v Dichloromethane