7PC7

The PDZ domain of SNTG1 complexed with the acetylated PDZ-binding motif of PTEN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	60.310, 61.740, 285.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.687 - 2.100
R-factor	0.1869
Rwork	0.185
R-free	0.21830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n7d 2pdz
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.687	2.150
High resolution limit [Å]	2.100	2.100
Rmeas	0.140	1.766
Number of reflections	63552	4649
<I/σ(I)>	14.38	1.71
Completeness [%]	100.0
Redundancy	13.36
CC(1/2)	0.999	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris 8.5, 22 % v/v PEG Smear Broad