7PC6

DNA-binding domain of a p53 homolog from the hydrothermal vent annelid Alvinella pompejana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999987
Spacegroup name	P 1
Unit cell lengths	55.900, 69.985, 114.849
Unit cell angles	88.07, 89.92, 81.51

Refinement procedure

Resolution	46.700 - 1.920
R-factor	0.185582919887
Rwork	0.183
R-free	0.22536
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xwr
RMSD bond length	0.007
RMSD bond angle	0.831
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.740	1.950
High resolution limit [Å]	1.920	1.920
Rmerge	0.056	0.835
Number of reflections	121535	6032
<I/σ(I)>	12.5	1.7
Completeness [%]	92.5	93
Redundancy	3.7	3.8
CC(1/2)	0.998	0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Protein solution: 7.2 mg/ml in 25 mM HEPES, pH 7.5, 150 mM NaCl, 0.5 mM TCEP. Crystallization buffer: 22% PEG 3350, 0.2M MgCl2, 0.1M Tris, pH 8.3. Drop size: 300 nL (150 + 150). Crystals were cryoprotected with mother liquor supplemented with 23% ethylene glycol.