7PC1

DNA binding domain of partition protein StbA of plasmid R388

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	105
Detector technology	CCD
Collection date	2009-05-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 31
Unit cell lengths	45.623, 45.623, 34.393
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.940 - 1.900
R-factor	0.203
Rwork	0.200
R-free	0.22220
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.923
Data reduction software	XDS
Data scaling software	SCALA (3.3.21)
Phasing software	PHENIX (1.18_3855)
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.400	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.032	0.386
Number of reflections	6294	897
<I/σ(I)>	28.5
Completeness [%]	99.3
Redundancy	5.7
CC(1/2)	0.999	0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M Trisodium Citrate Dihydrate pH5.6, 10% (v/v) iso-propanol and 10% (v/v) PEG 4000