7PBK

Vibriophage phiVC8 family A DNA polymerase (DpoZ), two conformations: thumb-exo open and thumb-exo closed

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-18
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.6926
Spacegroup name	P 21 21 2
Unit cell lengths	120.160, 158.440, 79.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.350 - 2.800
R-factor	0.1956
Rwork	0.192
R-free	0.25810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ktq
RMSD bond length	0.008
RMSD bond angle	0.955
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.350	2.870
High resolution limit [Å]	2.790	2.790
Rmerge	0.352	2.617
Rmeas	0.359	2.673
Rpim	0.069	0.529
Number of reflections	1019252	2621
<I/σ(I)>	9.7	1.4
Completeness [%]	99.4	92.7
Redundancy	26.5	23.5
CC(1/2)	0.996	0.413

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	300 mM ammonium citrate; 12% PEG 3350. Seeded from crystals grown at 291.15 K in 1 mM hexamine cobalt; 25% v/v isopropanol (100%); 100 mM HEPES pH 7