7PB9

Crystal structure of tandem WH domains of Vps25 from Odinarchaeota

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-24
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 2
Unit cell lengths	101.228, 31.461, 59.541
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.610 - 1.800
R-factor	0.2011
Rwork	0.198
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cuq
RMSD bond length	0.023
RMSD bond angle	2.013
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.610	1.850
High resolution limit [Å]	1.800	1.800
Rmeas	0.120	2.560
Number of reflections	18405	18405
<I/σ(I)>	10.3
Completeness [%]	100.0
Redundancy	12.7
CC(1/2)	1.000	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	12.5% PEG3350, 12.5% PEG1K, 12.5% MPD, 0.12 M Ethylene glycols, 0.1 M Buffer3 pH 8.5