7PB4

Cenp-HIK 3-protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	44.625, 56.119, 176.704
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.370 - 2.490
R-factor	0.2252
Rwork	0.222
R-free	0.28330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ypc
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.500	2.600
High resolution limit [Å]	2.490	2.490
Rmerge	0.130	0.700
Number of reflections	11771	11771
<I/σ(I)>	8.5
Completeness [%]	99.5
Redundancy	3.7
CC(1/2)	0.980	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	10% PEG 8k, 100 mM imidazole pH 8