7PAY

Structure of the human heterotetrameric cis-prenyltransferase complex in complex with magnesium and GGsPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000
Spacegroup name	H 3 2
Unit cell lengths	184.406, 184.406, 112.470
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.100 - 2.400
R-factor	0.2049
Rwork	0.202
R-free	0.24430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6z1n
RMSD bond length	0.004
RMSD bond angle	0.691
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.100	2.550
High resolution limit [Å]	2.400	2.400
Rmeas	0.116	0.983
Number of reflections	28670	8883
<I/σ(I)>	6.79
Completeness [%]	99.3
Redundancy	2.9
CC(1/2)	0.995	0.504

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	292	1 M LiSO4, 0.02 M Tris-HCl, 1.8% w/v PEG 8000