7PAA

JC polyomavirus VP1 in complex with scFv 29B1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	93
Detector technology	PIXEL
Collection date	2016-10-24
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	124.127, 172.530, 159.710
Unit cell angles	90.00, 104.53, 90.00

Refinement procedure

Resolution	48.507 - 3.102
Rwork	0.225
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nxg 4kq3
RMSD bond length	0.005
RMSD bond angle	1.296
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	3.180
High resolution limit [Å]	3.000	3.000
Rmeas	0.230	0.950
Number of reflections	129715	20801
<I/σ(I)>	10.37	2.21
Completeness [%]	99.8
Redundancy	7.06
CC(1/2)	0.990	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.2 M trimethanolamine N-oxide, 0.1 M Tris (pH 8.5), 20% PEG 2000