7P9M
BrxU, GmrSD-family Type IV restriction enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 196.875, 68.367, 129.591 |
| Unit cell angles | 90.00, 94.45, 90.00 |
Refinement procedure
| Resolution | 98.140 - 2.850 |
| R-factor | 0.2661 |
| Rwork | 0.266 |
| R-free | 0.27760 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.894 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (CCP4 Program Suite v7.1.015) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.140 | 2.960 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.044 | 1.021 |
| Number of reflections | 40622 | 4514 |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 1.9 | 2 |
| CC(1/2) | 0.999 | 0.368 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1 M Tris-HCl (pH 7.5), 0.2 M ammonium sulphate, 20% w/v PEG3350 |
