7P8E
Crystal structure of the Receiver domain of M. truncatula cytokinin receptor MtCRE1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9755 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 60.189, 60.189, 80.410 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 52.125 - 2.500 |
R-factor | 0.176 |
Rwork | 0.173 |
R-free | 0.22120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4euk |
RMSD bond length | 0.012 |
RMSD bond angle | 1.125 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3546) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.200 | 2.900 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.097 | 1.028 |
Rmeas | 0.106 | 1.129 |
Rpim | 0.042 | 0.456 |
Number of reflections | 3859 | 296 |
<I/σ(I)> | 12.9 | 2 |
Completeness [%] | 62.6 | 13.8 |
Redundancy | 6.2 | 6 |
CC(1/2) | 0.990 | 0.780 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | 0.1 M Tris (base), BICINE pH 8.5 0.03 MgCl2, 0.03 CaCl2 12.5% MPD, 12.5% PEG1000, 12.5% PEG 3350 |