7P80

Crystal structure of ClpP from Bacillus subtilis in complex with ADEP2 (compressed state)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-11
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97940
Spacegroup name	C 1 2 1
Unit cell lengths	108.617, 172.087, 84.137
Unit cell angles	90.00, 118.39, 90.00

Refinement procedure

Resolution	29.590 - 2.980
R-factor	0.2332
Rwork	0.229
R-free	0.27920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tt6
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.089
High resolution limit [Å]	2.980	2.982
Number of reflections	26196	2324
<I/σ(I)>	23.8
Completeness [%]	96.1
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	295	100 mM sodium acetate pH 4.6, 500 mM potassium thiocyanate