7P74

The PDZ domain of SYNJ2BP complexed with the phosphorylated PDZ-binding motif of RSK1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	59.620, 75.150, 108.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.707 - 1.900
R-factor	0.1825
Rwork	0.181
R-free	0.21070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n7d 2jik
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	1.950
High resolution limit [Å]	1.900	1.900
Rmeas	0.170	2.550
Number of reflections	39032	2825
<I/σ(I)>	11.16	1.46
Completeness [%]	99.5
Redundancy	13.3
CC(1/2)	0.999	0.593

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100mM ammonium sulfate, 100mM sodium formate, 25% PEG smear broad