7P73

The PDZ domain of SYNJ2BP complexed with the PDZ-binding motif of HTLV1-TAX1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	60.450, 63.990, 146.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.682 - 1.850
R-factor	0.1691
Rwork	0.168
R-free	0.19180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n7d 2jik
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	1.900
High resolution limit [Å]	1.850	1.850
Rmeas	0.142	2.290
Number of reflections	48765	3522
<I/σ(I)>	15.18	1.75
Completeness [%]	98.3
Redundancy	13.6
CC(1/2)	0.999	0.593

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	120mM Monosaccharides, 100mM Buffer System 3 pH 8.5, 50 % Precipitant Mix 2 (F10 Morpheus)