7P59

Variant Surface Glycoprotein 3 (VSG3, MiTat1.3, VSG224) with two O-linked post-translational modifications

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-16
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	I 21 3
Unit cell lengths	129.155, 129.155, 129.155
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.660 - 1.270
R-factor	0.1758
Rwork	0.175
R-free	0.19590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6elc
Data reduction software	xia2
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.660	1.318
High resolution limit [Å]	1.270	1.270
Rmerge	0.089	2.497
Rmeas	0.092	2.565
Rpim	0.020	0.585
Number of reflections	93285	9281
<I/σ(I)>	21.27	1.28
Completeness [%]	99.9	99.66
Redundancy	19.9	19.1
CC(1/2)	1.000	0.513

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	21% PEG 3350, 250mM NaCl, 100mM Tris, pH 8.2