7P4T

Tetrameric structure of murine SapA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 65
Unit cell lengths	48.489, 48.489, 314.359
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.400 - 3.170
Rwork	0.272
R-free	0.30400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ddj
RMSD bond length	0.003
RMSD bond angle	0.774
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.400	3.420
High resolution limit [Å]	3.170	3.170
Rmerge	0.178
Rpim	0.059	0.585
Number of reflections	5576	372
<I/σ(I)>	5.5	1.1
Completeness [%]	91.8
Redundancy	10.1
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	23.25% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.6