7P4G

Rabbit Muscle L-lactate dehydrogenase in complex with citrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-20
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.98
Spacegroup name	P 1
Unit cell lengths	83.705, 141.571, 148.136
Unit cell angles	114.55, 94.69, 102.20

Refinement procedure

Resolution	49.020 - 2.600
R-factor	0.2016
Rwork	0.199
R-free	0.25950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nqb
RMSD bond length	0.006
RMSD bond angle	1.422
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.020	2.660
High resolution limit [Å]	2.600	2.600
Number of reflections	162132	12112
<I/σ(I)>	5
Completeness [%]	93.4
Redundancy	2.1
CC(1/2)	0.970	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	10 % PEG 3350, 0.1 M Sodium Citrate